Upon equipping the rune, any non-respawning Hunter enemy or adversary will drop a Blood Dreg upon death, and after giving her one Blood Dreg, she will give you the "Deep Respect" gesture.
She is also present in Alfred's questline, and can be resurrected at the Altar of Despair once Alfred's questline has concluded.
Click to see dialogue
"Visitor… I claim no subjects, but here lieth Our throne. Kneel afore us… …or get thee gone."
Refuse to kneel
"Such impudence. Defiled, are we, yet still Queen. We shall not give audience to an ill-mannered beast. Get thee gone."
"Visitor… Moon-scented hunter… I am Annalise, Queen of Castle Cainhurst. Ruler of the Vilebloods, and sworn enemy of the church. Yet, our people are murdered, and we are prisoner to this wretched mask. What is it thou’rt in search of?"
Swear Vileblood oath
"Well, well… An odd hunter thou art indeed… We’ve tired of these piteous nights… Share in our plight, and take oath against the church. If thou wouldst this path walk… …I prithee partake of my rotted blood. Very well. Drink deep of our blood. Feel the spreading corruption burn. Now, thou’rt too a Vileblood. We two, the very last on this earth. We await thy return. For the honor of Cainhurst."
Refuse Vileblood oath
"A wise choice... There is no more to be said... away from mine gaze."
"Speak thy mind."
Refusing to kneel (swore oath)
"Arrant fool. Vileblood or no, forget not; We are thy Queen. Bend the knee."
Kneel (swore oath)
"Closest of kin, last of our kind. What is thy wish?"
"Speak not, those words. We have little need of a consort. Such a path would belike lead to further ruin. Thou'rt dear to us. We would see no harm befall thee..."
"Ahh, still thy honeyed tongue... The thought alone sufficeth. Thy worth is too great. Now, speak no more in the matter."
Click to see dialogue
”Is someone there? Well, whoever you are, it matters not. I will not die, tarnished as I am. You came here for naught. Be off with you."
"Cease this. I am Queen of the Vilebloods. The Healing Church binds our tongues. Now, off with you."
"You are a peculiar one. You’ve nothing to gain by speaking with me. Well, if you truly do not fear the Healing Church, tell me of your thoughts, your desires."
"I can help with other things, only no contracts. Anything that you wish. Anything at all."
"You are kind to me. You are… a friend. I will help you however I can."
"You are welcome here."
"What do you require? I am pleased to oblige."
"I see. Now, you must go. A blessing upon you, and your heartfelt kindness."
"Oh, dear me… have I offended you?"
"I am undead. There is nothing you can do to hurt me. They failed, and so will you."
The Ring of Betrothal unlocks a marriage proposal dialogue option when speaking to Annalise. However, she rejects the offer.
To unlock the dialogue "This chamber was not made for one alone", the player must perform chalice ritual with their own materials.
The Blood Dregs you give her resemble sperm cells. This is fitting, as she consumes them with the intent to bear a child.
Maybe after years of isolation, she grew tired of the "piteous nights" and that's why she suggest her "chamber was not made for one alone", which results in the hunter misunderstanding that she wants a consort.
Arianna'sdress is the same kind worn by Cainhurst nobility. It means she has some relation to Annalise.
The Queen is apparently immortal, as she can be revived even after Alfred brutally kills her, (using his Wheel to pound her into mush and paste, leaving small clumps of flesh and her head to remain) by taking the Queenly Flesh to the Altar of Despair. However since the Altar is improvised with the remains of what either is a being similar to Rom, or Rom herself and that the Queenly Flesh is offered to this altar, one can assume that there is an underlying connection between the Great Ones and the Vilebloods. This theory is further bolstered by the connection that Vilebloods have with the Pthumerians, as it is believed they are their descendants.
There is a picture of her holding a baby in the dining room.
Her character is based on the infamous historical serial killer, Elizabeth Báthory. The most prolific female murderer known to history, she is believed to have killed hundreds of girls. She was also accused of bathing in the blood (although this is agreed to be mere sensationalism) and was accused of forcing her servants to be stripped down and left in the cold, leaving them to die of hypothermia (which would explain the snowy location where the Vilebloods reside).
She possesses New Dialogue for the player in NG+ if you supported her or killed her.
She has a connection to Lady Maria, as she seems to be a distant relative, yet it can be assumed that they might have shared some degree of relationship (since they were indeed related) before the Church attacked the Vilebloods. This seems true as her attire was made specifically in Cainhurst and even wields the Evelyn, a weapon used by the knights of Cainhurst.